N Brookhouser*, T Nguyen*, SJ Tekel*, K Standage-Beier*, X Wang, DA Brafman (2020). CasMAS (XMAS)-TREE: A Cas9-Mediated Adenosine Transient Reporter for Editing Enrichment (CasMAsTREE). (In review). *co-first author
SJ Tekel, N Brookhouser, KA Haynes (2020). Delivery of cell-penetrating chromatin sensor-actuators to human osteosarcoma cells. March 2. Biorxiv, https://doi.org/10.1101/2020.02.28.969907
N Brookhouser*, SJ Tekel*, K Standage-Beier*, T Nguyen, G Schwarz, X Wang, DA Brafman. (2020). BIG-TREE: Base-edited isogenic hiPSC line generation using a transient reporter for editing enrichment. Stem Cell Reports, Volume 14, 11 February 2020, pg 184-191. *co-first author
K Standage-Beier*, SJ Tekel*, N Brookhouser*, G Schwarz, T Nguyen, X Wang, DA Brafman. (2019). A Transient Reporter for Editing Enrichment (TREE) in Human Cells. Nucleic Acids Research. doi: 10.1093/nar/gkz713. *co-first author
SJ Tekel, CL Smith, B Lopez, A Mani, C Connot, X Livingstone, KA Haynes (2019). Engineered orthogonal Quorum Sensing Systems for Synthetic Gene Regulation in Escherichia coli. Frontiers of Bioengineering and Biotechnology. 7:80.
R Daer, CM Barrett, EL Melendez, J Wu, SJ Tekel, J Xu, B Dennison, R Muller, KA Haynes (2018). Characterization of Diverse Homoserine Lactone Synthases in Escherichia coli. PloS One. 13(8): e0202294.
SJ Tekel, CM Barrett, DA Vargas, KA Haynes (2018). Design, construction, and validation of histone-binding effectors in vitro and in cells. ACS Biochemistry, Volume 57, Issue 31, 4707-4716.
SJ Tekel, DA Vargas, L Song, J LaBaer, MR Caplan, KA Haynes (2018). Tandem Histone-Binding Domains Enhance the Activity of a Synthetic Chromatin Effector. ACS Synthetic Biology, Volume 7, Issue 3, 12 February 2018, pg 842-852
SJ Tekel, KA Haynes (2017) Molecular Structures Guide the Engineering of Chromatin. Nucleic Acids Research, Volume 45, Issue 13, 27 July 2017, pg 7555-7570
Synthetic Transcriptional Regulator Compositions and Methods - Filed February 13, 2019. Application number: 16/274,770.
Transient Reporters and Methods for Base Editing Enrichment - Filed June 12, 2020. Application number: 63/038,220
Recent Conference Events
(2019) A Transient Reporter for Editing Enrichment (TREE) enables detection and isolation of single base edited cell lines-Engineering Biology Research Consortium (EBRC), Boston, MA
(2018) A Versatile Phenotypic Reporter for Cas9 Base Editing of Disease Relevant SNPs- Engineering Biology Research Consortium (EBRC), Fort Collins, CO
(2018) Generation of Disease Relevant Isogenic Stem Cell Lines Using Cas9 Base Editors – Synthetic Biology: Engineering, Evolution & Design (SEED), Scottsdale, AZ
(2018) Delivery of functional epigenetic regulators- Engineering Biology Research Consortium (EBRC), Seattle, WA